Antioxidant and antibacterial activity test on a crude extract of sand ( Holothuria atra) in Lemukutan water, Bengkayang District

Abstract

Sea cucumbers are soft-bodied marine invertebrates from the Holothuroidea class whose habitat is in sandy areas. This study aims to determine the content of secondary metabolites in crude extracts of sea cucumber which have the potential as antioxidants and antibacterials. The type of sea cucumber used in this research is the sand sea cucumber (Holothuria atra). The research was carried out from October 2020-February 2021. Samples were taken using the exploratory method, the sea cucumber samples were processed with 5 treatments, namely cleaning and crushing sea cucumber samples, extraction using the maceration method, evaporation with a vacuum rotary evaporator, and drying using freeze-drying. The bioactive components found in sea cucumbers are alkaloids, flavonoids, saponins, and phenols. The antioxidant activity found in sea cucumbers has an IC50 1194 ppm which is classified as a weak antioxidant. The antibacterial activity found intraday sand sea cucumbers E. coli gave the highest inhibition zone at a concentration of 20% with an inhibition zone diameter of 20.08 mm while S. aureus gave the highest inhibition zone at a concentration of 30% with an inhibition zone diameter of 22.17 mm.