Voltammetric determination of cefotaxime using potassium peroxomonosulfate

Abstract

The presented article is devoted to development of a new procedure for quantitative voltammetric determination of Cefotaxime powder for preparing the solution for injection in the form of the corresponding S-oxide in a weak acidic medium using potassium hydrogenperoxomonosulfate (KHSO 5) as an analytical reagent. Voltammogramms of Cefotaxime S-oxide solutions for different concentrations of cephalosporins have been scanned. There are two peaks on the voltammetric curve of the Cefotaxime S-oxide solution: at-0.65 V (that corresponds to potassium peroxomonosulfate) and-1.3 V (the peak height was rising proportionally to Cefotaxime concentrations increase) that has been chosen as analytical. The calibration curve method can be easily applied. Linearity has been studied over the drug concentration range from 1·10-4 to 1·10-3 mol L-1. The correlation coefficient is r = 0.999. Precision and accuracy have been studied by analyzing five replicates of the sample solutions at three concentrations levels. The relative standard deviations calculated were below 1.75%, d≤-1.1% indicating the excellent precision of the procedure proposed. The Limit of Detection (LOD) and the Limit of Quantification (LOQ) were calculated (LOD = 1.2·10-5 mol L-1 and LOQ = 4·10-5 mol L-1). The voltammetric method proposed is sensitive enough, accurate, precise, replicable and linear to enable determination of lower amounts of a drug. These advantages encourage the application of the method in the routine quality control of Cefotaxime in control and research laboratories.