Measuring Exercise Capacity and Physical Function in Adult and Older Mice

Abstract

The inability of older adults to maintain independence is a consequence of sarcopenia and frailty. In order to identify the molecular mechanisms responsible for decreased physical function, it will be critical to utilize a small animal model. The main purpose of this study was to develop a composite Comprehensive Functional Assessment Battery (CFAB) of well-validated tests to determine physical function and exercise capacity in 3 age groups of male C57BL/6 mice (6 months old, n = 29; 24 months old, n = 24; 28+ months old, n = 28). To measure physical function in mice, we used rotarod (overall motor function), grip meter (forelimb strength), treadmill (endurance), inverted cling (strength/endurance), voluntary wheel running (volitional exercise and activity rate), and muscle performance with in vivo contractile physiology (dorsiflexor torque). We hypothesized that CFAB would be a valid means to assess the physical function of a given mouse across the life span. In addition, we proposed that CFAB could be used to determine relationships between different parameters associated with sarcopenia. We found that there was an overall age-related significant decline (p