Cytoprotective activity of tomato and carrot callus on human dermal fibroblast adult (HDFa)

Abstract

Reactive oxygen species (ROS) exposure could lead to aging of the human cell. Tomato (Solanum lycopersicum L.) and carrot (Daucus carota L.) callus ethanolic extract are flavonoid-rich. Meanwhile tomato and carrot callus aqueous extract is suggested to contain protein that plays a role in cell regeneration against free radical. This study was aimed to evaluate the cytoprotective activity of tomato and carrot callus in preventing hydrogen peroxide (H 2 O 2 )-induced cell cycle arrest in G 0 /G 1 phase and inhibiting cell death. Cell death analysis of Human Dermal Fibroblast adult (HDFa) cells was performed using double staining method. Cell death inhibition showed in 0.15 mg · mL -1 and 0.25 mg · mL -1 of tomato callus ethanolic extract (TCEE); 0.15 mg · mL -1 and 1.0 mg · mL -1 of tomato callus aqueous extract (TCAE); 0.15 mg · mL -1 and 0.25 mg · mL -1 of carrot callus ethanolic extract (CCEE); 0.05 mg · mL -1 and 0.50 mg · mL -1 of carrot callus aqueous extract (CCAE) pre-treatment compared with H 2 O 2 control. The result of cell cycle analysis using FACS flows cytometry showed that 1.0 mg · mL -1 TCAE, 0.15 mg · mL -1 CCEE and 0.5 mg · mL -1 pre-treatment inclines to reduce accumulation of cell in G 0 /G 1 .