Preparation of Fermentation Medium of Rhizobium Meliloti Using Leaching Liquor of Corn Stalk

Abstract

In order to explore Rhizobium medium production using leaching liquor of green corn straw. Corn stalk powder extract solutions (CSE) are obtained and suitable carbon sources (glucose) were supplemented as liquid and solid Rhizobium medium. Compared with the yeast extract mannitol (YEM) medium, Rhizobium meliloti GN5 and Rhizobium meliloti 3436 are inoculated in two medium and viable cells after 30h fermentation were tested. Results show that nitrogen and carbon source in CSE medium can be utilized well by Rhizobium cells. Effect of CES-2 (ratio of green stalk powder and water is 1:50) of Rhizobium fermentation is better than that of YEM when additional glucose were supplemented. The growth of two Rhizobium colonies in CES-3 (the ratio of green corn stalk powder and water is 1:100) agar plate similar or big than that of YEM agar plate. The results suggest that suitable ratio green corn stalk extraction liquid with supplementation of additional carbon source can be used to product cheap and high quality medium for Rhizobium plate culture and fermentation.