Arbuscular mycorrhizal fungi can compensate for the loss of indigenous microbial communities to support the growth of liquorice (Glycyrrhiza uralensis fisch.)

Abstract

Soil microorganisms play important roles in nutrient mobilization and uptake of mineral nutrition in plants. Agricultural management, such as soil sterilization, can have adverse effects on plant growth because of the elimination of indigenous microorganisms. Arbuscular mycorrhizal (AM) fungi are one of the most important beneficial soil microorganisms for plant growth. However, whether AM fungi can compensate for the loss of indigenous microbial communities to support plant growth and metabolism is largely unknown. In this study, a pot experiment was conducted to investigate the effects of AM fungi on plant growth and secondary metabolism in sterilized and unsterilized soil. We used liquorice (Glycyrrhiza uralensis Fisch.), an important medicinal plant as the host, which was inoculated with the AM fungus Rhizophagus irregularis or not and grown in unsterilized or sterilized soil. Plant photosynthesis traits, plant growth and nutrition level, concentrations of the secondary metabolites, and expression levels of biosynthesis genes were determined. The results showed that soil sterilization decreased plant growth, photosynthesis, and glycyrrhizin and liquiritin accumulation, and moreover, downregulated the expression of related biosynthesis genes. Inoculation with R. irregularis in sterilized soil offset the loss of indigenous microbial communities, resulting in plant growth and glycyrrhizin and liquiritin concentrations similar to those of plants grown in unsterilized soil. Thus, AM fungi could compensate for the loss of indigenous microbial communities by soil sterilization to support plant growth and secondary metabolism.