An SECM detection scheme with improved sensitivity and lateral resolution: Detection of galactosidase activity with signal amplification by glucose dehydrogenase

Abstract

An enzymatic amplification cycle is used in a novel detection scheme for scanning electrochemical microscopy (SECM). An immobilized enzyme system comprising galactosidase and PQQ-dependent glucose dehydrogenase was imaged by SECM with high sensitivity and high lateral resolution. pQQ = pyrroloquinolinequinone.