Lentropin: A factor in vitreous humor which promotes lens fiber cell differentiation

Abstract

An activity has been identified in chicken vitreous humor which stimulates embryonic chicken lens epithelial cells to elongate and specialize for lens crystallin synthesis. The activity is heat-labile and is destroyed by treatment with trypsin or agents that reduce disulfides. Gel filtration and ultrafiltration analyses indicate that it has an apparent molecular weight of ≃60,000. Its properties differ from those of an activity present in serum which also can promote lens fiber cell formation in vitro. We call this material 'lentropin' and suggest that it is responsible for stimulating lens fiber cell formation in vivo and, consequently, plays an important role in determining the shape and polarity of the lens.