The Quality of Ettawa Crossbreed Sperm: The Treatment with a Combination of Cryoprotectant in Tris Diluents

Abstract

The formation of ice crystals easily damages the sperm of goats during the freezing process. This study aimed to determine the optimum treatment of cryoprotectant DMSO, glycerol, and a combination of DMSO with glycerol in Tris-egg yolk diluents to maintain the quality of frozen sperm of ettawa breeds. This study used four male goats of which semen was collected twice a week using an artificial vagina. The independent variables in this study were 6% DMSO, 6% glycerol, and a combination of 3% DMSO: 3% glycerol, negative control (without treatment), and positive control (7% glycerol). The sperm quality parameters include motility, viability, and abnormalities. Data collection techniques used observation techniques before and after freezing. The data were tested statistically using one-way ANOVA and Duncan test. The results showed that the combination treatment of 3% DMSO: 3% glycerol produced significant motility, viability, and abnormalities (P <0.05) compared to other treatments. These results indicated that the addition of a combination of DMSO and glycerol in egg yolk thinners successfully protected sperm from various stresses during the cryopreservation process to maintain the sperm quality.