In silico Characterization of the Structure of Genes and Proteins related to ?-carotene Degradation in Musa acuminata DH-Pahang and Musa balbisiana Pisang Klutuk Wulung

Abstract

?-carotene is an important nutritional content in banana. However, its lifetime depends on the enzymes controlling its conversion into strigolactone. To understand the involved enzymes activity, which are ?-carotene isomerase (D27), carotenoid cleavage dioxygenase 7 (CCD7), and CCD8, would be the key to manipulate the rate of ?-carotene degradation. In this research, the structure of genes and proteins of the D27, CCD7, and CCD8 from Musa acuminata were characterized. DH-Pahang and Musa balbisiana Pisang Klutuk Wulung (PKW). The corresponding sequence of genes from both species were aligned to determine similarity and intron/exon positions. Domains and motifs in the sequences of putative proteins of D27, CCD7, and CCD8 were also identified. It was found that D27, CCD7, and CCD8 genes in DH-Pahang and PKW comprise of various nucleotide sequence length, putative proteins, and numbers and length of exons and introns. However, the putative proteins possess the same domains: DUF4033 (domain of unknown function) in D27 and RPE65 (retinal pigment epithelium) in CCD7 and CCD8. Phylogenetic trees showed that D27, CCD7, and CCD8 proteins from DH-Pahang and PKW are conserved and clustered in the same clades with the same proteins of monocot plants. Hence, the results could be useful for future research in optimizing ?-carotene content in banana.