The Effect of Electric Cigarette Exposure on The Histopathology of Pulmo in Albino Rats (Rattus norvegicus)

Abstract

This study aimed to identify the pulmo histopathology figure of albino rats exposured to an e-cigarette. A total of 20 rats divided in 5 groups, control group (K) were not exposed by e-cigarette and treatment group were exposed by e-cigarette in different doses, P1 (0,3mg/ml), P2 (3mg/ml), P3 (12 mg/ml), P4 (36 mg/ml) respectively. After 14 days of treatment, rats were euthanasia and necropsy method was performed to identify macroscopy change, also the pulmo organs were dissected and preserved for histopathological study using Hematoxilin-Eosin staining. The results showed that damage of pulmo was defected within histopathology examination using 400x magnification. Moreover, the data were analyzed using Kruskal-Wallis test and continued by Mann Whitney Test which s how a significant difference (p<0.05). Average result from pulmo organs of Rats were Control (K)=3.00; (P1)=6.00; (P2)=12.00; (P3)=13.00 and (P4)=18.50 and nicotine dose of minimum was 3 mg/ml. In conclusion, the exposure of an e-cigarette have significant damage effect of pulmo organs in rats.