Compensating Light Intensity Attenuation in Confocal Scanning Laser Microscopy by Histogram Modeling Methods

Abstract

The scientific discipline of microscopy aims to make possible the visualization of objects that cannot be observed by the unassisted human vision system, allowing researchers to enhance their understanding on the morphology and processes which characterize such objects. All microscopy techniques by themselves represent crucial tools for scientists working in various fields of research, and furthermore, when combined with image processing and computer vision algorithms the level of information and the speed at which it can be extracted from microscopy images can be greatly increased.