Isolation and identification of iridoid from water extract of leaves of vitex pinnata linn. As antioxidant

Abstract

An iridoid compound with antioxidant activity was isolated from the water extract of leaves of Halban (Vitex pinnata Linn.). Isolation and identification processes of the compound were performed based on the bioassay-activity-guided method using the free radical scavenging method for antioxidant. The purification was done by the column chromatography analysis [(SiO2; (i) dichloromethane:methanol = 5:1–1:1, (ii) dichloromethane:methanol:water = 5:5:1, (iii) dichloromethane:methanol = 5:1)] to give white powder. Chemical structure determination was done by an interpretation of spectroscopy data (UV, IR, NMR and MS) and revealed a glycoside iridoid compound with the high antioxidant activity (IC50 27.3 ppm).