Crystallization and preliminary X‐ray crystallographic studies on Type III antifreeze protein

Abstract

Type III antifreeze protein, more specifically the recombinant QAE‐Sephadex‐binding isoform, has been crystallized in 50–55% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0–4.5. The resultant crystals belong to the ortho‐rhombic space group P212121 with a = 32.60 Å, b = 39.00 Å, and c = 46.57 Å and diffract to at least 1.7 Å. A set of 1.7‐Å native data has been collected, with completeness 93.4% and Rsvm of 0.069. Initial screening for heavy‐atom derivatives has yielded a Pt‐bound derivative. Copyright © 2000 The Protein Society