Chromatography of Acidic Polysaccharides on Deae-Cellulose

Abstract

The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid polysaccharides, e.g. pectic acid, were eluted by various strengths of alkali. Neutral polysaccharides could also be hromatographed at a somewhat alkaline pH by working with borate systems, which give charged complexes with many carbohydrates.