Efficacy of root-associated fungi and PGPR on the growth of Pisum sativum (cv. Arkil) and reproduction of the root-knot nematode Meloidogyne incognita

Abstract

The effects of root-associated fungi (Aspergillus awamori and Glomus mosseae) and plant growth promoting rhizobacteria (PGPR) (Pseudomonas putida, Pseudomonas alcaligenes and Paenibacillus polymyxa) were studied alone and in combination in glasshouse experiments on the growth of pea, enzyme activity (peroxidase and catalase) and reproduction of root-knot nematode Meloidogyne incognita. Application of A. awamori, G. mosseae and PGPR caused a significant increase in pea growth and enzyme activities of both nematode inoculated and uninoculated plants. A. awamori was more effective in reducing galling and improving the growth of nematode inoculated plants than P. alcaligenes or P. polymyxa. The greatest increase in growth, enzyme activities of nematode-inoculated plants and reduction in galling and nematode multiplication was observed when A. awamori was used with P. putida or G. mosseae as compared to the other combinations tested. Percentage root colonization was higher when AM fungus inoculated plants were treated with P. putida both in presence and absence of nematode. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.