Phosphorus retention in a lowland Neotropical stream following an eight-year enrichment experiment

Abstract

Human alteration of the global P cycle has led to widespread P loaDing in freshwater ecosystems. Much research has been devoted to the capacity of wetlands and lakes to serve as long-term sinks for P inputs from the watershed, but we know much less about the potential of headwater streams to serve in this role. We assessed storage and retention of P in biotic and abiotic compartments after an 8-y experimental P addition to a 1st-order stream in a Neotropical wet forest. Sediment P extractions indicated that nearly all P storage was in the form of Fe-and Al-bound P (?700 ?g P/g dry sediment), similar to nearby naturally high-P streams. At the end of the enrichment, ?25% of the total P added over the 8-y study was still present in sediments within 200 m of the injection site, consistent with water-column measurements showing sustained levels of high net P uptake throughout the experiment. Sediment P declined to baseline levels (?100 ?g P/g dry sediment) over 4 y after the enrichment ended. Leaf-litter P content increased nearly 2× over background levels during P enrichment and was associated with a 3× increase in microbial respiration rates, although these biotic responses were low compared to nearby naturally high-P streams. Biotic storage accounted for <0.03% of retention of the added P. Our results suggest that the high sorption capacity of these sediments dampened the biotic effects of P loaDing and altered the timing and quantity of P exported downstream.