Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

T. D. Hua; V. Rolland-Fulcrand; R. Lazaro; P. Viallefont; M. P. Lefranc; M. Weill

Journal ArticleOPEN ACCESS

Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

Tetrahedron Letters (1996) 37(2) 175-178

DOI: 10.1016/0040-4039(95)02122-1

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Abstract

A very low level of enzyme activity (10-11 mol) has been detected using a PCR method following a vector hybridisation of a supported oligonucleotide. This methodology could be very useful for direct active catalytic antibody screening from a combinatorial library.

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Hua, T. D., Rolland-Fulcrand, V., Lazaro, R., Viallefont, P., Lefranc, M. P., & Weill, M. (1996). Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies. Tetrahedron Letters, 37(2), 175–178. https://doi.org/10.1016/0040-4039(95)02122-1

Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

Abstract

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