A comparison of the bactericidal activity of quinolone antibiotics in a Mycobacterium fortuitum model

Abstract

New agents are urgently needed to meet the threat of multiple drug-resistant tuberculosis and to manage infection with the naturally resistant non-tuberculosis mycobacteria. Earlier finoroqninolones have been shown to have promising in-vitro activity, although mouse infection and clinical studies suggested that they lack sufficient bactericidal activity. Methods were evaluated to measure the bactericidal activity of fluoroqinolones and to compare the new agent moxifloxacin with other fouroquinolones with M. fortuitum as a model system. The optimum bactericidal concentrations (OBC) for the fouroquinolones were: Moxifioxacin, 0.5 mg/L; ciprofioxacin and sparfloxacin, 2mg/L and ofloxacin, 8mg/L. The bactericidal indices (BI) for moxifloxacin, ciprofloxacin, sparfloxacin and ofloxacin were 1.8, 0.5, 0.2 and 0.2, respectively. Similar ranking was obtained when the time taken to produce one log10 reduction in viable count was calculated. These data indicate that moxifloxacin was the most bactericidal of the flouroquinolones tested. Such methods provide a simple in-vitro measure that correlates with in-vivo models.