Chemical constituents from Chinese mangrove plant Cerbera manghas

Abstract

Objective: To investigate the chemical constituents of mangrove plant Cerbera manghas collected in Hainan Province of China. Methods: Column chromatography techniques were used for separation and purification of the compounds and extensive spectral analyses including 2D NMR spectrum were employed for structural elucidation. Results: Seven compounds were obtained from leaves of C. manghas, including 3R, 5S, 6S, 7E, 9S-3, 5, 9-trihydroxy-6-methoxy-7-megastigmane (I), blumenol A (II), 3R, 5S, 6R, 7E, 9S-3, 5, 6, 9-tetrahydroxy-7-megastigmane (III), 12β-hydroxyl-5α-pregnane-16-ene-3, 20-dione (IV), 12β-hydroxyl-pregnane-4, 16-diene-3, 20-dione (V), 3β, 12β-dihydroxy-pregnane-16-ene-20-one (VI), 3a, 12β-dihydroxy-pregnane-16-ene-20-one (VII). Conclusion: Compound I is a new compound named as euscaphin B and other compounds are isolated from C. manghas for the first time.