Chemical constituents of Tongmai formula.

Abstract

Objective: To study the chem. constituents of Tongmai formula and set an example for its bioactive substances foundation investigation. Method: The chem. constituents were isolated and purified by column chromatog. of macroporous resin, silica gel, Sephadex LH-20 and reversed phase high-performance liq. chromatog., and their structures were identified by physico-chem. properties and spectrum anal. Result: Thirty-four compds. were sepd. and identified as tanshinone I (1), cryptotanshinone (2), dihydrotanshinone I (3), formononetin (4), protocatechuic aldehyde (5), daidzein (6), trans-ferulic acid (7), ononin (8), 5-hydroxyononin (9), senkyunolide H (10), (4R)-3-[2-hydroxy-4-methoxyphenyl]-4-(4-β-D-glucopyranosyloxybenzyl)-but-2-en-4-olide (pueroside D, 11), genistin (12), daidzin (13), 3'-methoxypuerarin (14), formononetin-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (15), formononetin-7-O-β-D-apiofuranosyl-(1→6)-O-β-D)-glucopyranoside (16), lanceolarin (17), kakkanin (18), biochanin A-8-C-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (19), puerarin (20), sucrose (21), adenosine (22), mirificin (23), 3'-hydroxypuerarin (24), 3'-methoxydaidzein-7,4'-di-O-β-D-glucopyranoside (25), daidzein-7,4'-O-glucoside (26), 3'-methoxydaidzin (27), formononetin-8-C-β-D-xylopyranosyl-(1→6)-O-β-D-glucopyranoside (28), genistein-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (ambocin, 29), genistein-8-C-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (30), daidzein-7-O-β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranoside (31), daidzein-7-O-β-D-glucopyranosyl-(1→4) -O-β-D-glucopyranoside (32), 3'-hydroxymirificin (33), 6'-O-D-xylosylpuerarin (34). Conclusion: On the basis of the above mentioned results and previously reported chem. studies on the Radix Puerariae, Salvia miltiorrhiza, and Ligusticum chuanxiong, it was suggested that the main chem. components were isoflavonoid compds. coming from the Radix Puerariae, and the secondary components were tanshinones coming from the Salvia miltiorrhiza, and senkyunolide H coming from the Ligusticum chuanxiong in the Tongmai formula. Compds. 16, 17, and 29 were isolated from Radix Puerariae and the genus Pueraria DC. for the first time. [on SciFinder(R)]