The role of cucumic melo varieta agrestis, Vitamin C and zinc on sperm activity and oxidative stress during cryopreservation in infertile male

Abstract

The objective of this study was to investigate the role of Cucumic melo varagrestis, Vitamin C and zinc on human spermatozoa and oxidative stress during cryopreservation in asthenozoospermia. One hundred males were participated in this study at Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/Al- Nahrain University. There ages were (31.86 ±0.76) years. Semen samples were collected and seminal fluid analysis was done according to WHO (1999). Cryopreservation kept for one month in liquid nitrogen (LN2) at (-196◦C 1ml semen sample with 1ml of sperm freeze medium with zinc, vitamin c, cucmic melon extraction). After one month; 0.5 ml of Ferticuilt Flushin was added to pallet and incubation 37◦C\30min and Microscopic sperm parameters Examination had conducted. The oral administration of antioxidant combination of Cucumic melo var. agrestis, vitamin C and zinc improves sperm characteristics in asthenozoospermia.