Contribución a la Caracterización y Evaluación de la Actividad Antioxidante de las Antocianinas del Fruto de Ivilan (Monnina Obtusifolia H.B.K)

Abstract

Through the use of different chromatographic techniques extracts rich in polyphenols (anthocyanins) were obtained. From the purified extracts, the main anthocyanins of the Monnina Obtusifolia fruit were identified for the first time: one pyroanthocyanin and three anthocyanins derived from delphinidin, cyanidin and peonidin. The relative contribution of different types of fractions isolated from the fruit (raw extracts, purified and pure compounds) on their antioxidant properties was also evaluated. The anthocyanin with greater power to capture the synthetic radical ABTS.+ was the compound derived from delphinidin. It was found that aglycones with only one OH group in the B ring (4'-OH) have lower antioxidant activity; compared to compounds possessing 3 ', 4' di-OH substituted group such as delphinidin and cyanidin derivatives. The analysis of the reducing capacity of Folin-Ciocalteau and the antioxidant capacity equivalent to trolox of the isolated fractions suggest that the fruit of Monnina Obtusifolia can be considered as an important source of bioactive compounds, for its use in the industry.