Tissue Engineered Osteogenesis in Bone Defects by Homologous Osteoblasts Loaded on Sterile Bioresorbable Coral Scaffold in Rabbits

Abstract

Objectives: This study explores feasibility of tissue-engineered osteogenesis using sterile coral implants loaded with homologous osteoblasts to repair bone defects. Study Design: A unilateral 4 mm transverse discontinuity defect was produced approximately mid-way along left radius of young female rabbits using rotary diamond disc under continuous saline irrigation and stabilised with autoclaved steel miniplate and screws. The defect was then fitted with sterile bioresorbable coral implant loaded with homologous neonatal calvarial osteoblasts or control implants without osteoblasts. All animals underwent radiography immediately post-operative, at weekly intervals for four weeks and at fortnightly intervals thereafter. Operated bones were histologically evaluated for osteogenesis at 12 weeks. Results: Findings demonstrate osteogenesis and complete repair of bioresorbable coral implant by homologous osteoblasts loaded on coral scaffold. Conclusions: Single stage surgery using this technique to induce osteogenesis and closure of discontinuity bone defects including palatal clefts and peripheral reduction of large craniofacial defects might prove better therapeutic modality than autologous bone grafting or tissue distraction osteogenesis. [PUBLICATION ABSTRACT]