Correlation between hypoxia inducible factor-1α and vesicular endothelial growth factor in male wistar rat brain tissue after anaerobic exercise

Abstract

During physical activity, oxygen reduction leading to hypoxia affects brain cell metabolisms, induces stabilization of Hypoxia Inducible Factor-1α (HIF-1α) and up-regulate Vesicular Endothelial Growth Factor (VEGF). This study aimed to investigate the correlation of HIF-1α and VEGF of brain tissue in male wistar rat after anaerobic exercise. Twenty four rats were divided into four groups: control, 1x, 3x and 7x in a week of anaerobic exercise. A rat treadmill was used at speed 35 m min-1, 20 min for every anaerobic exercise. The HIF-1α and VEGF level were measured by ELISA. The correlation between HIF-1α and VEGF was analyzed using Pearson test. The HIF-1α and VEGF level increased in 1x and 3x a week of anaerobic exercise. The highest HIF-1α and VEGF level were observed in 1x a week of anaerobic exercise. Pearson test between HIF-1α and VEGF showed r = 0.709 and p = 0.000. These findings showed that HIF-1α and VEGF are correlated and anaerobic exercise affects HIF-1α and VEGF level.