Evaluation of the role of rhizobacteria in controlling root-knot nematode infection in Lycopersicon esculentum plants by modulation in the secondary metabolite profiles

Abstract

Environmental stress imposes negative impacts on the growth and development of the crop plants. The present study was designed to assess the effect of plant growth-promoting rhizobacteria (PGPR) (Pseudomonas aeruginosa and Burkholderia gladioli) on plant pigments and phenolic compounds in 10-day-old root-knot nematode (RKN)-infected Lycopersicon esculentum seedlings. The levels of different osmoprotectants and organic acids were also evaluated in nematode-infected L. esculentum seedlings. Our results revealed that nematode-infected seedlings had reduced levels of plant pigments (chlorophyll (70.5 %), carotenoids (64.8 %) and xanthophylls (34.3 %)) and enhanced levels of phenolic compounds (total phenols (40.3 %), flavonoids (80.9 %), anthocyanins (28.9 %) and polyphenols (366.1 %)), osmoprotectants (total osmolytes (15.3 %), total carbohydrates (54.9 %), reducing sugars (45.3 %), trehalose (94.5 %), glycine betaine (59.01 %) and proline (69.6 %)) and (citric acid (28.4 %), fumaric acid (18.16 %), succinic acid (179.9 %) and malic acid (21.7 %)). The levels of these metabolites increased after inoculation with P. aeruginosa and B. gladioli. The expression of genes encoding different enzymes pertaining to phenols and organic acid metabolism was also studied. The expression of genes was elevated in nematode-infected plants, i.e. CHS (chalcone synthase) by 1.32-folds, PAL (phenylalanine ammonia lyase) by 1.16-folds, CS (citrate synthase) by 1.6-folds, SUCLG1 (succinyl-CoA ligase) by 1.19-folds, SDH (succinate dehydrogenase) by 1.92-folds, FH (fumarate hydratase) by 2.4-folds and malate synthase (MS) by 1.26-folds and further upregulated after PGPR inoculation. This study demonstrates the importance of PGPR in managing nematode infection in plants through alteration in the synthesis of different secondary metabolites in plants.