Caracterização da variabilidade de genes relacionados à fertilidade de machos e ao temperamento em eqüinos da raça brasileira Mangalarga

Abstract

The aims of the present study were to propose a PCR-RFLP genotyping method for the AJ-315378:c.110A>G and AB-264325:c.771G>C SNPs in the equine CRISP1 and HTR1A genes, respectively, as well as to characterize these and another polymorphism, AB-098561:c.1470G>A of the SLC6A4 gene, in order to provide a basis for future studies investigating the association between DNA markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. PCR-RFLP was found to be adequate for the genotyping of the SNPs AJ-315378:c.110A>G of the CRISP1 and AB-264325:c.771G>C of the HTR1A. However, the polymorphism of the CRISP1 probably does not occur in Mangalarga horses, a fact impairing association studies of this marker with traits related to male fertility. The estimative of the population genetic parameters obtained for the polymorphisms AB-264325:c.771G>C of the HTR1A and AB-098561:c.1470G>A of the SLC6A4 in the studied sample discourage the conduct of research addressed the association between markers and traits related to temperament.