Microwave-Assisted Synthesis, Antioxidant and Toxicological Evaluation of a Hydrazone, 1-(4-chlorobenzylidene)-2-phenylhydrazine

Abstract

Hydrazone is an imine-derived compound that has various biological activities such as anticancer, antitumor, antioxidant, antimicrobial, and antidepressant. Antioxidants play an important role in removing oxidants and preventing cell damage and the toxicity of a compound is one of the parameters in drug discovery, especially anticancer. In this work, a hydrazone, 1-(4-chlorobenzylidene)-2-phenylhydrazine has been synthesized under microwave irradiation and evaluated for its antioxidant activity by DPPH assay. The toxicity of this compound was evaluated by Brine Shrimp Lethality Test (BSLT) for pre-screening of anticancer drugs. The compound was elucidated for its structure using UV-Vis, FTIR, GC-MS, 1D, and 2D NMR spectrometers. Based on the toxicological evaluation using BSLT, the synthesized compound possessed a highly toxic with LC50 value of 0.018 μg/mL and has strong antioxidant activity with IC50 value of 41.33 μg/mL. These suggest that the synthesized hydrazone compound is a potential compound in the development of antioxidant and anticancer drugs.