Antimicrobial Production Using Endophytic Fungus Sporothrix sp. LBKURCC43 by Carbon and Nitrogen Modification

Abstract

Endophytes are endosymbiont organisms that can produce antimicrobial compounds. Media composition is one of the main components to produce industrial compounds. This study investigated the optimal media composition for antimicrobial production by endophytic fungus Sporothrix sp. LBKURCC43. The media were modified by applying composition combination between corn, potato, and sweet potato as carbon sources, and soybean and beef meat as nitrogen sources; incubation times of the fermentation were 5, 10, 15, 20, and 25 days. Antibacterial activity against pathogenic microorganisms (Escherichia coli, Staphylococcus aureus, and Candida albicans) was examined by using the agar diffusion method. A phytochemical test was conducted for determining the highest antibacterial activity. The results showed compounds produced in modified medium MM 03 (potato and soybean) had the highest inhibition zone diameter (8.09 ± 0.62 mm) at 20 days of incubation against E. coli (the ratio between potato and soybean for the medium was 3.60: 0.24). While MM 05 (corn, potato, and soybean at a ratio of 1.8: 1.8: 0.24) indicated the highest inhibition zone against S. aureus (6.03 ± 0.82 mm) at day 20. None of the compound in the modified medium was active against C. albicans. In conclusion, there were varies abilities of endophytic fungus in producing antimicrobial compounds based on their growth medium composition. The highest activity against E. Coli was obtained in the medium consisted of potato and soybean. The phytochemical test indicated the modified medium (MM 05) contained a compound that was categorized as saponin.