The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2 v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
CITATION STYLE
dos Santos, E. O., Michelon, M., Furlong, E. B., Burkert, J. F. de M., Kalil, S. J., & Burkert, C. A. V. (2012). Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Brazilian Journal of Microbiology, 43(2), 432–440. https://doi.org/10.1590/S1517-83822012000200002
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