Isolation and characterization of cDNA encoding the gamma-subunit of cGMP phosphodiesterase in human retina

Abstract

Cyclic GMP-phosphodiesterase (cGMP-PDE) plays a key role in the normal functioning of retinal rod photoreceptor cells. The enzyme is composed of α- and β-catalytic which are inhibited by two identical γ-subunits. A cDNA encoding the γ-subunits (PDEγ) from human retina has been cloned and sequenced. The 1012-bp cDNA has a coding region of 261 bp which is high homologous to those of the PDEγcDNAs from bovine and mouse retinas. Comparison of the deduced amino acid sequences of the proteins from the three species indicates that PDEγ has been very well conserved through evolution. The mRNA encoded by the cloned cDNA is 1.0 kb long, is similar in size to the corresponding mRNAs from mouse, dog and bovine retinas and is not detected in ground squirrel retina. The PDEG gene has been assigned to human chromosome 17, probably in the region q21.1. © 1990.