Optimization of FPase activity using sorghum straw planted in malaysia by aspergillus terreus SUK-1 via solid substrate fermentation

Abstract

The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL-1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL-1 which indicate the validity of the model.