Determination of (-)-borneol, camphor and isoborneol in Blumea balsamifera (L.) DC. Leaves by simultaneous ultrasonic and microwave assisted extraction and gas chromatography

Abstract

In present work, simultaneous ultrasonic and microwave assisted extraction followed by GC-FID was developed for quantitative analysis of the bioactive components of (-)-borneol, camphor and isoborneol in Blumea balsamifera leaves. After systematical investigation, the optimal experimental parameters microwave power (100 W) and extraction time (30 s) were investigated. The optimized method provided satisfactory linearity, precision, stability and recovery. The proposed method was applied for determination of three target compounds in leaves samples from 14 different times of harvest period. The variations of three target compounds were monitored and the result demonstrates (-)-borneol content of leaves was higher level from mid-October to next January. It has been shown that the proposed ultrasonic and microwave assisted extraction-GC-FID is a simple, rapid and reliable method for quantitative analysis of (-)-borneol, camphor and isoborneol in B. balsamifera leaves and a potential tool for quality assessment of B. balsamifera leaves.