Suppression of electron transfer to dioxygen by charge transfer and electron transfer complexes in the FAD-dependent reductase componentof toluene dioxygenase

Abstract

Background: Toluene dioxygenase reductase converts the redox equivalents of NADH under oxic conditions. Results: Reduced reductase forms a stable complex with NAD+ that shows slower electron transfer to dioxygen. Conclusion: Charge transfer complex formation regulates the reactivity of the reductase. Significance: Charge transfer complexes between flavins and nicotinamides are abundant and likely to have diverse mechanistic functions. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.