Detection of Cryptosporidium parvum using a specific polymerase chain reaction

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Abstract

The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys. © 1993.

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Webster, K. A., Pow, J. D. E., Giles, M., Catchpole, J., & Woodward, M. J. (1993). Detection of Cryptosporidium parvum using a specific polymerase chain reaction. Veterinary Parasitology, 50(1–2), 35–44. https://doi.org/10.1016/0304-4017(93)90005-8

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