P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

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Abstract

Summary: The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. Availability and implementation: The P-SAMS website is available at http://p-sams.carringtonlab.org.

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Fahlgren, N., Hill, S. T., Carrington, J. C., & Carbonell, A. (2016). P-SAMS: A web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics, 32(1), 157–158. https://doi.org/10.1093/bioinformatics/btv534

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