Fermentation Process of Glycerol to Arabitol from Byproducts of Reutalis trisperma Biodiesel Using Yeast of Debaryomyces Hansenii

Abstract

Arabitol byproduct on the production of biodiesel Reutalis trisperma has calories of 0.2 cals / g equivalent to the level of sweetness of sucrose can be produced by using the yeast of Debaryomyces hansenii. The research was done by experimental method and descriptive analysis with two replications. The variation in this study was the ratio between the concentration of glycerol substrate and the fermentation medium added to 250 m / L of work volume in five treatments, namely: A (1: 16) crude glycerol, B (1: 16) pure glycerol, C (1: 8) crude glycerol, D (1:5) crude glycerol, and E (1:4) crude glycerol. The experimental results that the D. hansenii ITB CC R85 yeast may accumulate the formation of arabitol during the stationary phase at 120 h. The best fermentation condition was obtained at treatment C with a specific growth rate (μ) of 0.0608/h, arabitol concentration of 0.2373 g / L, the yield of arabitol to glycerol (Yp / s) of 0.0041 g / g, the yield of biomass to the substrate (Yx / s) of 0.3214 g / g.