Electrochemical detection of vascular endothelial growth factor with aptamer sandwich

Abstract

We developed an electrochemical vascular endothelial growth factor (VEGF) detection system using two VEGFbinding aptamers. Sensitive detection systems of VEGF are highly required for cancer diagnosis. In this study, VEGF-A was detected using a sandwiching method with a pyrroquinoline quinone glucose dehydrogenase (PQQGDH)-labeled VEGF-binding aptamer and another aptamer immobilized onto a gold wire electrode. We evaluated various combinations of VEGF-binding aptamers to be employed in the process. In addition, we examined dose dependency for the electric current generated by PQQ-GDH, measured in the constructed VEGF detection system. Using this newly constructed system, we successfully detected VEGF 165at 15 nM (M = mol dm -3) concentration. © The Electrochemical Society of Japan.