Comparison of ECL, ELISA and ELFA immunoassays

Abstract

The aim of these study was the evaluation of electrochemiluminescence assay (ECL), enzyme-linked immunosorbent assay (ELISA) and enzyme-linked fluorescent assay (ELFA), for an early diagnosis of Cytomegalovirus infections in pregnant women. In medical diagnostics, it is necessary to determine the most sensitive techniques for the detection of this pathogen, due to it’s multiple fetal infections during pregnancy. The ECL technique resulted with the highest sensitivity and specificity values. However, for diagnostic purposes, the results should always be assessed in conjunction with the patient’s medical history and other clinical examinations.