Antagonism of Siderophore Producing Bacteria against Blood Disease Bacteria

Abstract

Siderophore is a chemical compound of Fe chelating. This compound is produced by several microorganisms that grow under iron-limiting conditions. Siderophore can facilitate the transfer of Fe from the environment to become available to plants. The siderophore ability to bind Fe as a competitor against other microorganisms, so that in the agricultural system it can be used as a plant disease controller. This study aims to determine the production of siderophore produced by several fluorescent pseudomonad isolates and its antagonistic tests against Blood Disease Bacteria (BDB). Siderohore detection is determined by the absorbance value obtained, and measured using a spectrophotometer at a wavelength of 410 nm. The antagonist test used a completely randomized designwith 7 treatments and 3 replications. The treatment is fluorescent pseudomonads isolates PfLAHP2, PfPb1, PfCas3, PfKd7, PfCas, PfPj1, and PfPj2. PfLAHP2 isolates produced the highest siderophore, which was 1.027, and the lowest isolate PfCas was 0.148. The antagonistic test of fluorescent pseudomonad against BDB showed that PfLAHP2 isolate produced the largest inhibitory zone, which was 1.042 cm.