Photochemical ligation of DNA probe prepared in click chemistry

Abstract

Template-directed photoligation with 5-carboxyvinyl-2'-deoxyuridine (CVU) can be used for genomic research, biotechnology and biomedical agents. However, in photoligation with CVU, a long photoirradiation time at 366 nm is required to complete photoligation. To overcome the limitation of photoligation with CVU, we synthesized new photosensitive probes in the copper-catalyzed azide-alkyne cycloaddition reaction, as the best example of click chemistry. © 2009CPST.