Protein phosphatase 2A and Cdc7 kinase regulate the DNA unwinding element-binding protein in replication initiation

Abstract

Background: Cdc7 kinase and protein phosphatase 2A are important in DNA replication initiation. Results: DNA unwinding element (DUE)-binding protein (DUE-B) is an essential replication factor that is regulated by Cdc7 and PP2A. Conclusion: The phosphorylation of DUE-B determines its ability to bind the minichromosome maintenance helicase and regulate Cdc45 chromatin loading. Significance: PP2A and Cdc7 regulate DUE-B mediated loading of Cdc45 to the prereplication complex.