Prediction of first hyperpolarizability of fluorescent proteins

Abstract

In an attempt to investigate the predictability of nonlinear optical properties of fluorescent proteins, we have experimentally and theoretically determined the first hyperpolarizability of eGFP, eYFP, ZsYellow, DsRed, mStrawberry and mCherry. Our first comparisons between experiment and calculations meet our expectations and point out some key features, e.g. the strong dependence on X-ray data and sensitivity to environmental factors like pH and ionic strength.