Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (105/mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.
CITATION STYLE
Barbosa, J., Rodrigues, A. G., Pérez, M. J., & Pina-Vaz, C. (2014). Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry. Memorias Do Instituto Oswaldo Cruz, 109(8), 1078–1080. https://doi.org/10.1590/0074-0276140267
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