Identifikasi Spesies Ikan Laut Menggunakan Teknik DNA Barcoding

Abstract

Biodiversity research, especially marine biodiversity in Indonesia, is one of the crucial research topics. This is due to Indonesia's high biodiversity which requires supporting research, such as species identification, population status, and also genetic stock using a molecular approach. This research was conducted from May to August 2023. A total of six individual samples were collected from three locations in Karangasem Regency, Bali, namely Amed, Batu Dawa, and Batuniti. This research aims to report the results of activities carried out with the theme of identifying fish species using a molecular approach. DNA replication was carried out using the Polymerase Chain Reaction (PCR) method targeting the COI (Cytochrome Oxidase Subunit I) gene. The types of fish obtained included four individuals of tuna (Auxis rochei), one pompano (Caranx sp.), and one keel-jawed needlefish (Tylosurus acus). The analysis results show that all samples are closely related to the NCBI comparison results, namely Auxis rochei, Caranx sexfasciatus, and Tylosurus acus with similarity percentage values ranging from 98-100%.