Ad-shWnt2b vector therapy demonstrates antitumor activity in orthotopic intrapleural models as monitored with the in vitro imaging system (IVIS)

Abstract

Aim: We investigated whether Wnt2B-inhibiting therapy has effective antitumor activity against Wnt2B-overexpressing cells in an orthotopic intrapleural model by monitoring with the in vitro imaging system (IVIS). Materials and Methods: Mice for the orthotopic intrapleural model were randomized into 3 groups (control, Ad-shWnt2B and Ad-scramble; 8 mice per group). The respective vector was injected into the intrapleural cavity of mice in the Ad-shWnt2B and Ad-scramble groups. After 6 weeks of vector treatment, all mice were monitored with the IVIS. Additionally, their body weight was measured until all mice died from the tumor or were sacrificed. Results: A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively).