Morelloflavone and Molecular Docking from Stembark of Chisocheton lasiocarpus and Its Cytotoxic Activity Against Breast Cancer Mcf-7 Cell Lines

Abstract

Chisocheton is a plant of the Meliaceae family which has been known as a source of limonoids, triterpenoids, steroids, alkaloids, and phenolics. This plant is the second largest in the Meliaceae, with 53 species widely distributed in tropical and subtropical regions including Indonesia. From this genus, compounds that have interesting activities have been found, including anti-inflammatory, antimalarial, cytotoxic, antitumor, and anticancer. One of the species that has the potential to discover new compounds is Chisocheton lasiocarpus because there are still few phytochemical studies. This study aims to obtain compounds that are active against MCF-7 breast cancer cells using the insilico method against ER-α receptors. The morelloflavone compound was isolated from the bark of C. lasiocarpus using extraction and partitioning techniques which were then separated using column chromatography using the eluent n-hexane: dichloromethane: ethyl acetate (2:3:5). The pure isolates were then tested for their potential anticancer activity against MCF-7 cells and in silico testing for ER-α receptors (PDB code: 3ERD), ER-β (PDB code: 1QKM) and HER-2 (PDB code: 3PP0).