The β5-loop and lid domain contribute to the substrate specificity of pancreatic lipase-related protein 2 (PNLIPRP2)

Abstract

Background: Two pancreatic lipases differ in substrate specificities. Results: Substitution of specific surface loops from one lipase into the other decreases activity against triglycerides and galactolipids. Conclusion: The β5-loop and lid domain influence lipase substrate specificity. Significance: Defining the relationship of structure to lipase function is crucial for understanding lipolysis and developing reagents to modulate lipase activity.