Regulation of DARPP-32 phosphorylation by three distinct dopamine D 1-like receptor signaling pathways in the neostriatum

Abstract

Dopamine D1-like receptors play a key role in dopaminergic signaling. In addition to Gs/olf/adenylyl cyclase (AC)-coupled D 1 receptors, the presence of D1-like receptors coupled to Gq/phospholipase C (PLC) has been proposed. Benzazepine D1 receptor agonists are known to differentially activate Gs/olf/AC and Gq/PLC signaling. By utilizing SKF83959 and SKF83822, we investigated the D1-like receptor signaling cascades, which regulate DARPP-32 phosphorylation at Thr34 (the PKA-site) in mouse neostriatal slices. Treatment with SKF83959 or SKF83822 increased DARPP-32 phosphorylation. The SKF83959- and SKF83822-induced increase in DARPP-32 phosphorylation was largely, but partially, antagonized by a D1 receptor antagonist, SCH23390, and the residual SCH23390-insensitive increase was abolished by an adenosine A2A receptor antagonist. In addition, the SKF83959-induced, SCH23390-sensitive increase in DARPP-32 phosphorylation was enhanced by a PLC inhibitor. Analysis in slices from D1R/D2R-DARPP-32 mice revealed that both D1 receptor agonists regulate DARPP-32 phosphorylation in striatonigral, but not in striatopallidal, neurons. Thus, dopamine D1-like receptors are coupled to three signaling cascades in striatonigral neurons: (i) SCH23390-sensitive Gs/olf/AC/PKA, (ii) adenosine A2A receptor-dependent Gs/olf/AC/PKA, and (iii) Gq/PLC signaling. Interestingly, Gq/PLC signaling interacts with SCH23390-sensitive Gs/olf/AC/PKA signaling, resulting in its inhibition. Three signaling cascades activated by D1-like receptors likely play a distinct role in dopaminergic regulation of psychomotor functions. © 2008 The Authors.