Evaluation and comparison of recombinant Omp 28 antigen in indirect ELISA for sero-prevalence study of canine brucellosis

Abstract

Brucella melitensis rec. Omp 28 was evaluated and compared as antigen in I-ELISA with HSE and SA as well as with RBPT. Dog serum samples (787) were collected and tested. A sero-prevalence of 12.3,8.3,7.6 and 4.3% of canine brucellosis was recorded by rec. Omp ELISA, SA ELISA, RBPT, HSE ELISA, respectively. Significant differences were observed between sero-prevalence rates estimated by all tests except between SA ELISA and RBPT. The rec. Omp ELISA had the highest relative sensitivity while HSE ELISA exhibited the maximum relative specificity in comparison with RBPT. The present study advocated to use rec. Omp 28 antigen based ELISA for diagnosis of canine brucellosis.